Phytosanitary Control
The phytosanitary control of RGV from genebank collection

The main activities of this compartment are:

  • the monitoring health status of regenerated and multiplied germplasm, in the experimental field;
  • the phytosanitary control of seeds samples introduced in the genebank collection.

The monitoring health status of the multiplied genetic material is carried out in different vegetation periods (Photo 1) starting from the appearance of the first symptoms until maturity, in the case of diseases (Photo 2), and at pest from the sings' occurrence of attacks (Photo 3). The phytosanitary status monitoring of multiplied/regenerated plant species is made through the use of same descriptors from the inspection sheets, in according to the operational guidelines issued by the genebank. The plots with multiplied/regenerated samples should be evaluated as:

  • admitted samples;
  • Samples damaged by diseases and pest that require the application of chemical treatments (Photo 4);
  • attacked samples in high percentage (over 90%) that will be removed.
Photo 1. The health status monitoring in the experimental field
Photo 2. The anthracnose (Ascochyta fabae) attack of faba bean
Photo 3. The pest attack on the eggplant leafs
Photo 4. The application of chemical treatments during the vegetation period

The phytosanitary control of seeds samples that will be stored in genebank collection from distinct sources (multiplication/regeneration, collecting, acquisitions) is carried out in the laboratory through different types of tests (Photo 5,6,7,8) that establish the health status of the seeds. After processing, the samples are assigned by curators at the phytopathology laboratory, in order to examine the health status (according to operational guide edited by genebank).

The tested samples are evaluated in this way:

  • admitted samples, on the labels are noted: "phytosanitary certified, the date and signature of the person responsible" (Photo 9);
  • samples damaged by pests (seeds are treated with fumigants (Photo 10));
  • samples with few seeds damaged by diseases (it is recommended multiplication in the experimental field);
  • samples attacked by diseases and pests in the percentage of 5% (it is recommended to remove them).
Photo 5. The macroscopic appearance of Fusarium graminearum, on the seeds
Photo 6. The tested seeds on absorbent paper
Photo 7. The tested seeds on potato dextrose agar medium
Photo 8. Microscopic identify of the seed-borne pathogens
Photo 9. Image with seed samples notation mode phytosanitary certified
Photo 10. Treated seeds with fumigants

We collect, study and preserve plant genetic diversity for present and future utilization.