Regeneration & multiplication of seeds is required when the germination percentage has fallen below acceptable level, or seeds number is under agreed standard by genebanks.
As a rule, the germination percentage must be more than 85%, except for samples where the treatments are ineffective (such as wild species), when germination is allowed between 60% and 80%.
Photo 2. Multiplication plot of some bean local varieties
Photo 3. Multiplication plots of some peppers and tomatoes local varieties
The evaluation compartment works with a large number of species that have divided in varied crop groups. For most species, there are multiplication & regeneration guides which were edited by Suceava Genebank. Seeds multiplication & regeneration is performed in the experimental field (1ha) and in two unheated greenhouses (0,50ha)
For achieving of the germplasm regeneration and multiplication in the adequate conditions we have to take into considerations the following points:
- Selection within the original population as a result of regeneration procedures should be minimized;
- The population size of the original sample should be sufficiently large to avoid genetic drift;
- Cross pollinating crops need additional measures to insures proper isolation;
- No contamination with other samples during regeneration and seed handling should be allowed;